ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	FL83B cells (1 × 105) were cultured on to 6 well plate and maintained in complete media for 12 hours. The cells were then transfected with ON-TARGETplus SMARTpool siRNA targeting STAT5b or ON-TARGETplus Non-targeting siRNA (Dharmacon, Chicago) using Fugene-6 (Roche, IN) according to the manufacturer’s instruction. Following transfection with this mixture for 24 h, cells were treated with 40 mM MSM for an additional 24 h. Following this, total proteins were prepared and the expression levels of ANGPTL3 and STAT5b were analyzed using western blotting.

Protocol tips

FL83B cells (1 × 105) were cultured on to 6 well plate and maintained in complete media for 12 hours. The cells were then transfected with ON-TARGETplus SMARTpool siRNA targeting STAT5b or ON-TARGETplus Non-targeting siRNA (Dharmacon, Chicago) using Fugene-6 (Roche, IN) according to the manufacturer’s instruction. Following transfection with this mixture for 24 h, cells were treated with 40 mM MSM for an additional 24 h. Following this, total proteins were prepared and the expression levels of ANGPTL3 and STAT5b were analyzed using western blotting.

Publication protocol

FL83B cells (1 × 105) were cultured on to 6 well plate and maintained in complete media for 12 hours. The cells were then transfected with ON-TARGETplus SMARTpool siRNA targeting STAT5b or ON-TARGETplus Non-targeting siRNA (Dharmacon, Chicago) using Fugene-6 (Roche, IN) according to the manufacturer’s instruction. Following transfection with this mixture for 24 h, cells were treated with 40 mM MSM for an additional 24 h. Following this, total proteins were prepared and the expression levels of ANGPTL3 and STAT5b were analyzed using western blotting.

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