Stealth siRNA(m) Atg16l2

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	For silencing of Atg16l2, islolated mouse pancreatic acinar cells were transfected with Atg16l2 siRNA (MSS232103; Invitrogen) and control siRNA (12935-113; Invitrogen). Atg16l2 ON-TARGET SMARTpool from Dharmacon and siCONTROL Non-Targeting Pool were also used. Transfections (100 pmoles of each siRNA) were performed using the Amaxa Nucleofactor Electroporation System. For silencing of p62, isolated mouse pancreatic acinar cells were infected with lentiviruses encoding p62 shRNA (generated in our laboratory) for 2 days with polybrene, and knock down efficiency was examined by RT-PCR or IB analysis. We used the following p62 (m) siRNA sequence: 5′-CCGCATCTACATTAAAGAGAA-3′.

Protocol tips

For silencing of Atg16l2, islolated mouse pancreatic acinar cells were transfected with Atg16l2 siRNA (MSS232103; Invitrogen) and control siRNA (12935-113; Invitrogen). Atg16l2 ON-TARGET SMARTpool from Dharmacon and siCONTROL Non-Targeting Pool were also used. Transfections (100 pmoles of each siRNA) were performed using the Amaxa Nucleofactor Electroporation System. For silencing of p62, isolated mouse pancreatic acinar cells were infected with lentiviruses encoding p62 shRNA (generated in our laboratory) for 2 days with polybrene, and knock down efficiency was examined by RT-PCR or IB analysis. We used the following p62 (m) siRNA sequence: 5′-CCGCATCTACATTAAAGAGAA-3′.

Publication protocol

For silencing of Atg16l2, islolated mouse pancreatic acinar cells were transfected with Atg16l2 siRNA (MSS232103; Invitrogen) and control siRNA (12935-113; Invitrogen). Atg16l2 ON-TARGET SMARTpool from Dharmacon and siCONTROL Non-Targeting Pool were also used. Transfections (100 pmoles of each siRNA) were performed using the Amaxa Nucleofactor Electroporation System. For silencing of p62, isolated mouse pancreatic acinar cells were infected with lentiviruses encoding p62 shRNA (generated in our laboratory) for 2 days with polybrene, and knock down efficiency was examined by RT-PCR or IB analysis. We used the following p62 (m) siRNA sequence: 5′-CCGCATCTACATTAAAGAGAA-3′.

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