siRNA(r) NM_057107 Acsl3

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

Protocol tips

Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

Publication protocol

Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

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