siRNA(r) NM_012820 Acsl1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

Protocol tips

Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

Publication protocol

Rat Acsl1, Acsl3, and Acsl4 siRNAs (20 nM) (siRNA ID numbers 178 SASI_Rn01_00073026, SASI_Rn01_00100113, and SASI_Rn01_00041844; 179 Sigma) or rat cPLA2α siRNA (5 nM) (siRNA ID numbers SASI_Rn02_ 180 00267738) as well as negative control siRNA (Sigma) were transfected 181 into 3Y1 cells using Lipofectamine RNAiMAX reagent according to the 182 manufacturer's instructions. Two days after transfection, the media 183 were replaced with phenol red-free DMEM supplemented with 2% FCS. 184 After 24 h culture, 1 ng/ml IL-1β was added to the culture to activate 185 the cells. The efficacy of each siRNA was assessed by real-time PCR or 186 immunoblotting.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Rat - 3Y1 Acsl1 using siRNA(r) NM_012820 Acsl1 from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for siRNA(r) NM_012820 Acsl1 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Rat - 3Y1 Acsl1 using siRNA(r) NM_012820 Acsl1 from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.