Rn_Ywhaz_4 FlexiTube siRNA

Protocol tips

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	"H9c2 rat cardiac-derived myoblasts (ATCC) were cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin at 37 C, 5% CO2. Cells transfected with negative control siRNA (Invitrogen), SIRT2 siRNA (Dharmacon – Cat. No. M-08072-00), 14-3-3 f siRNA (Qiagen – Cat. No. SI02055536), pcDNA3.1 plasmid (Invitrogen), pcDNA-FLAG/HA-14-3-3 f (Addgene), pcDNA3.1-Myc/His-SIRT2 construct (Biochain) or with the deacetylase mutant SIRT2 construct pcDNA-SIRT2 (N168A) [8] using a Nucleofector II Device (Amaxa) were cultured for 48 h prior to experiments"

Protocol tips

"H9c2 rat cardiac-derived myoblasts (ATCC) were cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin at 37 C, 5% CO2. Cells transfected with negative control siRNA (Invitrogen), SIRT2 siRNA (Dharmacon – Cat. No. M-08072-00), 14-3-3 f siRNA (Qiagen – Cat. No. SI02055536), pcDNA3.1 plasmid (Invitrogen), pcDNA-FLAG/HA-14-3-3 f (Addgene), pcDNA3.1-Myc/His-SIRT2 construct (Biochain) or with the deacetylase mutant SIRT2 construct pcDNA-SIRT2 (N168A) [8] using a Nucleofector II Device (Amaxa) were cultured for 48 h prior to experiments"

Publication protocol

H9c2 rat cardiac-derived myoblasts (ATCC) were cultured in DMEM containing 10% FBS, 1% penicillin/streptomycin at 37 C, 5% CO2. Cells transfected with negative control siRNA (Invitrogen),
SIRT2 siRNA (Dharmacon – Cat. No. M-08072-00), 14-3-3 f siRNA (Qiagen – Cat. No. SI02055536), pcDNA3.1 plasmid (Invitrogen), pcDNA-FLAG/HA-14-3-3 f (Addgene), pcDNA3.1-Myc/His-SIRT2 construct (Biochain) or with the deacetylase mutant SIRT2 construct pcDNA-SIRT2 (N168A) [8] using a Nucleofector II Device (Amaxa) were cultured for 48 h prior to experiments

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Manufacturer protocol

Download the product protocol from Qiagen for Rn_Ywhaz_4 FlexiTube siRNA below.

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