FITC Annexin V Apoptosis Detection Kit I

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 3×105 cells were seeded per dish. - FC101 (0–5 µM) is treated on cells for 72 h.	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Upstream tips
- 3×105 cells were seeded per dish. - FC101 (0–5 µM) is treated on cells for 72 h.
Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Cells were seeded in 60-mm dishes at a density of 3×105 cells per dish in the growth medium and grown overnight at 37°C in a humidified incubator with 5% CO2. Following treatment with FC101 (0–5 µM) for 72 h, the cells (including floating and attached cells) were harvested, followed by apoptosis assay using the Annexin V-FITC Apoptosis Detection Kit I (BD Biosciences, San Jose, CA), according the manufacturer’s instructions. Flow cytometry was performed using a FACSCalibur flow cytometer (Becton Dickinson). Cells treated with vehicle alone (PBS) served as a control.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I below.

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