ON-TARGETplus Rat Lsm1 (364624) siRNA - SMARTpool

Protocol tips

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	Cells were transfected with 1 µg of plasmid DNA in 12-well tissue culture plate format. Plasmids that expressed short hairpin RNA (shRNA) against specific transcripts were similarly transfected by using 1 µg DNA and induced with 300 ng/ml doxycycline. On-Target Plus SmartPool small interfering RNAs (siRNAs) against Dcp1a and Lsm1 were obtained from Dharmacon (Lafayette, CO, USA). Tet-stable PC12 cells were transfected with inducible plasmids and induced with 500 ng/ml doxycycline medium for 24 or 48 h. PC12 cells were transfected by using LipofectamineLTX (Thermo Fisher Scientific). All transfections were performed according to the manufacturer’s protocol.

Protocol tips

Cells were transfected with 1 µg of plasmid DNA in 12-well tissue culture plate format. Plasmids that expressed short hairpin RNA (shRNA) against specific transcripts were similarly transfected by using 1 µg DNA and induced with 300 ng/ml doxycycline. On-Target Plus SmartPool small interfering RNAs (siRNAs) against Dcp1a and Lsm1 were obtained from Dharmacon (Lafayette, CO, USA). Tet-stable PC12 cells were transfected with inducible plasmids and induced with 500 ng/ml doxycycline medium for 24 or 48 h. PC12 cells were transfected by using LipofectamineLTX (Thermo Fisher Scientific). All transfections were performed according to the manufacturer’s protocol.

Publication protocol

Cells were transfected with 1 µg of plasmid DNA in 12-well tissue culture plate format. Plasmids that expressed short hairpin RNA (shRNA) against specific transcripts were similarly transfected by using 1 µg DNA and induced with 300 ng/ml doxycycline. On-Target Plus SmartPool small interfering RNAs (siRNAs) against Dcp1a and Lsm1 were obtained from Dharmacon (Lafayette, CO, USA). Tet-stable PC12 cells were transfected with inducible plasmids and induced with 500 ng/ml doxycycline medium for 24 or 48 h. PC12 cells were transfected by using LipofectamineLTX (Thermo Fisher Scientific). All transfections were performed according to the manufacturer’s protocol.

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