Flot2 Rat siRNA Oligo Duplex (Locus ID 83764)

Protocol tips

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	Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

Protocol tips

Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

Publication protocol

Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

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Manufacturer protocol

Download the product protocol from OriGene for Flot2 Rat siRNA Oligo Duplex (Locus ID 83764) below.

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