Flot1 Rat siRNA Oligo Duplex (Locus ID 64665)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

Protocol tips

Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

Publication protocol

Seventy-two hours after NRCM isolation, cells were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) with either 50 nM siRNA against Flotillin-1 ((ID 64665) Trilencer-27 Rat siRNA (SR514199), Origene Technologies, Inc.), 20 nM siRNA against Flotillin-2 ((ID 83764) Trilencer-27 Rat siRNA (SR 500907), Origene Technologies, Inc.) or both. Transfection with 10 nM scrambled siRNA (Trilencer-27 Universal Scrambled Negative Control siRNA duplex (SR30004)) and non-transfected cells were used as controls. From every experiment, a few wells were used to validate the efficiency of transfection by Western Blot (see Figure 4A).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing siRNA / miRNA gene silencing Rat - NRCM Flot1 using Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) from OriGene.

Manufacturer protocol

Download the product protocol from OriGene for Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing siRNA / miRNA gene silencing Rat - NRCM Flot1 using Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) from OriGene. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.