Stealth siRNA(r)_BIRC3

siRNA / miRNA gene silencing Rat - B35 cIAP2/BIRC3

Experiment
siRNA / miRNA gene silencing Rat - B35 cIAP2/BIRC3
Product
Stealth siRNA(r)_BIRC3 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Transfection of siRNA was done as described previously [24]. Commercially available small interfering RNA (siRNA) duplexes directed against rat cIAP1 and cIAP2 were purchased from Invitrogen (Carlsbad, California) as described [25], and are all selected and validated Stealth siRNAs. Control siRNA duplexes were purchased from Dharmacon (Lafayette, CO). Lamin A/C siRNA (Dharmacon) was used to optimize the transfection conditions and efficiency. Additional controls of nonspecific siRNA duplex or vehicle alone were evaluated and found not to alter cIAP-1 and cIAP-2 mRNA and protein expression at the concentration of siRNA found to reduce the specific target message and protein optimally.

Publication protocol

Transfection of siRNA was done as described previously [24]. Commercially available small interfering RNA (siRNA) duplexes directed against rat cIAP1 and cIAP2 were purchased from Invitrogen (Carlsbad, California) as described [25], and are all selected and validated Stealth siRNAs. Control siRNA duplexes were purchased from Dharmacon (Lafayette, CO). Lamin A/C siRNA (Dharmacon) was used to optimize the transfection conditions and efficiency. Additional controls of nonspecific siRNA duplex or vehicle alone were evaluated and found not to alter cIAP-1 and cIAP-2 mRNA and protein expression at the concentration of siRNA found to reduce the specific target message and protein optimally.

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Manufacturer protocol

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