Stealth siRNA(r)_BIRC2

Protocol tips

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	Transfection of siRNA was done as described previously [24]. Commercially available small interfering RNA (siRNA) duplexes directed against rat cIAP1 and cIAP2 were purchased from Invitrogen (Carlsbad, California) as described [25], and are all selected and validated Stealth siRNAs. Control siRNA duplexes were purchased from Dharmacon (Lafayette, CO). Lamin A/C siRNA (Dharmacon) was used to optimize the transfection conditions and efficiency. Additional controls of nonspecific siRNA duplex or vehicle alone were evaluated and found not to alter cIAP-1 and cIAP-2 mRNA and protein expression at the concentration of siRNA found to reduce the specific target message and protein optimally.

Protocol tips

Transfection of siRNA was done as described previously [24]. Commercially available small interfering RNA (siRNA) duplexes directed against rat cIAP1 and cIAP2 were purchased from Invitrogen (Carlsbad, California) as described [25], and are all selected and validated Stealth siRNAs. Control siRNA duplexes were purchased from Dharmacon (Lafayette, CO). Lamin A/C siRNA (Dharmacon) was used to optimize the transfection conditions and efficiency. Additional controls of nonspecific siRNA duplex or vehicle alone were evaluated and found not to alter cIAP-1 and cIAP-2 mRNA and protein expression at the concentration of siRNA found to reduce the specific target message and protein optimally.

Publication protocol

Transfection of siRNA was done as described previously [24]. Commercially available small interfering RNA (siRNA) duplexes directed against rat cIAP1 and cIAP2 were purchased from Invitrogen (Carlsbad, California) as described [25], and are all selected and validated Stealth siRNAs. Control siRNA duplexes were purchased from Dharmacon (Lafayette, CO). Lamin A/C siRNA (Dharmacon) was used to optimize the transfection conditions and efficiency. Additional controls of nonspecific siRNA duplex or vehicle alone were evaluated and found not to alter cIAP-1 and cIAP-2 mRNA and protein expression at the concentration of siRNA found to reduce the specific target message and protein optimally.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Stealth siRNA(r)_BIRC2 below.

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