ON-TARGETplus Rat Tmed10 (84599) siRNA - SMARTpool

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	Tmp21 was depleted by two sequential knockdowns within 48 hr using 50 nM concentration of ON-TARGETplus SMARTpool Rat Tmed10 siRNA and Dharmafect 2 transfection reagent (Dharmacon), and experiments were performed 24 hr after the second knockdown. Tmp21 was depleted by two sequential knockdowns using ON-TARGETplus SMARTpool Rat Tmed10 siRNA (Dharmacon L-092937-01-0005) and Dharmafect 2 transfection reagent (Dharmacon T-2002-02) exactly as recommended by the manufacturer. The second knockdown was performed 48 hr after the first, and experiments were performed 24 hr after the second knock down.

Protocol tips

Tmp21 was depleted by two sequential knockdowns within 48 hr using 50 nM concentration of ON-TARGETplus SMARTpool Rat Tmed10 siRNA and Dharmafect 2 transfection reagent (Dharmacon), and experiments were performed 24 hr after the second knockdown. Tmp21 was depleted by two sequential knockdowns using ON-TARGETplus SMARTpool Rat Tmed10 siRNA (Dharmacon L-092937-01-0005) and Dharmafect 2 transfection reagent (Dharmacon T-2002-02) exactly as recommended by the manufacturer. The second knockdown was performed 48 hr after the first, and experiments were performed 24 hr after the second knock down.

Publication protocol

Tmp21 was depleted by two sequential knockdowns within 48 hr using 50 nM concentration of ON-TARGETplus SMARTpool Rat Tmed10 siRNA and Dharmafect 2 transfection reagent (Dharmacon), and experiments were performed 24 hr after the second knockdown. Tmp21 was depleted by two sequential knockdowns using ON-TARGETplus SMARTpool Rat Tmed10 siRNA (Dharmacon L-092937-01-0005) and Dharmafect 2 transfection reagent (Dharmacon T-2002-02) exactly as recommended by the manufacturer. The second knockdown was performed 48 hr after the first, and experiments were performed 24 hr after the second knock down.

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Rat Tmed10 (84599) siRNA - SMARTpool below.

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