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siRNA smart pools for Pak1 (L-091307-02), Trio (L-087912-01), RhoG (L-083717-02), β-Pix (L-098943-02) and α-Pix (L-098102-02) and single oligonucleotides for Rac1 (LQ-080171-02), Pak1 (LU-091307-02) and Trio (LU-087912-02) were purchased from Dharmacon (Thermo Scientific) or Qiagen (Rac1: 5′-AAAGAGAUCGGUGCUGUCAAA-3′; ref. 28) and transfected with Oligofectamine (Invitrogen) |
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Protocol tips |
siRNA smart pools for Pak1 (L-091307-02), Trio (L-087912-01), RhoG (L-083717-02), β-Pix (L-098943-02) and α-Pix (L-098102-02) and single oligonucleotides for Rac1 (LQ-080171-02), Pak1 (LU-091307-02) and Trio (LU-087912-02) were purchased from Dharmacon (Thermo Scientific) or Qiagen (Rac1: 5′-AAAGAGAUCGGUGCUGUCAAA-3′; ref. 28) and transfected with Oligofectamine (Invitrogen) |
Publication protocol
siRNA smart pools for Pak1 (L-091307-02), Trio (L-087912-01), RhoG (L-083717-02), β-Pix (L-098943-02) and α-Pix (L-098102-02) and single oligonucleotides for Rac1 (LQ-080171-02), Pak1 (LU-091307-02) and Trio (LU-087912-02) were purchased from Dharmacon (Thermo Scientific) or Qiagen (Rac1: 5′-AAAGAGAUCGGUGCUGUCAAA-3′; ref. 28) and transfected with Oligofectamine (Invitrogen)
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Manufacturer protocol
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