ON-TARGETplus Rat Prkaa2 (78975) siRNA - SMARTpool

siRNA / miRNA gene silencing Rat - UMR‐106 Ampkα2/Prkaa2

Experiment
siRNA / miRNA gene silencing Rat - UMR‐106 Ampkα2/Prkaa2
Product
ON-TARGETplus Rat Prkaa2 (78975) siRNA - SMARTpool from Horizon Discovery Ltd.
Manufacturer
Horizon Discovery Ltd.

Protocol tips

Protocol tips
total of 1.5X 105 cells were seeded 24 hours before the experiment in antibiotic-free medium. Cells were transfected with ONTARGETplus Rat SMARTpool Ampka1 and Ampka2 siRNA (each 50 nmol/l) (Thermo Fisher Scientific, Waltham, MA) or 100 nmol/l nonspecific siRNA (Thermo Fisher Scientific) using DharmaFECT1transfection reagent. Forty-eight hours after transfection, cells were treated with 1,25(OH)2D3 (100 nmol/l) for another 24 hours and harvested.

Publication protocol

total of 1.5X 105 cells were seeded 24 hours before the experiment in antibiotic-free medium. Cells were transfected with ONTARGETplus Rat SMARTpool Ampka1 and Ampka2 siRNA (each 50 nmol/l) (Thermo Fisher Scientific, Waltham, MA) or 100 nmol/l nonspecific siRNA (Thermo Fisher Scientific) using DharmaFECT1transfection reagent. Forty-eight hours after transfection, cells were treated with 1,25(OH)2D3 (100 nmol/l) for another 24 hours and harvested.

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Papers

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Rat Prkaa2 (78975) siRNA - SMARTpool below.

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