ON-TARGETplus Rat Pld2 (25097) siRNA - SMARTpool

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	The PLD2 siRNA used was ON-TARGET plus SMART pool L-089287-00-0010 (Dharmacon—Thermo Fisher Scientific, Lafayette, CO). 0.8 x 105 RBL-2H3 cells/well were plated in 6 well plates for 16 h. Transfections were performed using Lipofectamine RNAiMAX (Life Technologies) with 20nM PLD2 siRNA. After 3 days RNA was extracted to verify the PLD2 knockdown level. As a negative control siRNA ON-TARGET plus Non-targeting (Dharmacon) was used

Protocol tips

The PLD2 siRNA used was ON-TARGET plus SMART pool L-089287-00-0010 (Dharmacon—Thermo Fisher Scientific, Lafayette, CO). 0.8 x 105 RBL-2H3 cells/well were plated in 6 well plates for 16 h. Transfections were performed using Lipofectamine RNAiMAX (Life Technologies) with 20nM PLD2 siRNA. After 3 days RNA was extracted to verify the PLD2 knockdown level. As a negative control siRNA ON-TARGET plus Non-targeting (Dharmacon) was used

Publication protocol

The PLD2 siRNA used was ON-TARGET plus SMART pool L-089287-00-0010 (Dharmacon—Thermo Fisher Scientific, Lafayette, CO). 0.8 x 105 RBL-2H3 cells/well were plated in 6 well plates for 16 h. Transfections were performed using Lipofectamine RNAiMAX (Life Technologies) with 20nM PLD2 siRNA. After 3 days RNA was extracted to verify the PLD2 knockdown level. As a negative control siRNA ON-TARGET plus Non-targeting (Dharmacon) was used

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