Silencer® Select_Mapk14/p38 siRNA(r)

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	Primary neonatal rat ventricular myocyte (NRVM) cultures were prepared from 1–2-day-old rats using standard methods as previously described [10]. Cells were cultured in growth medium consisting of 10% Horse Serum (HS), 5% Fetal Bovine Serum (FBS) and 1% Penicillin/Streptomycin (PS) in F-10 Nutrient Mixture Media. The experiments were carried 36 h after cells were seeded. Pre-designed siRNA targeting rat p38 mRNA and an siRNA control were obtained from Invitrogen (siRNA p38 s135447 and siRNA control AM4611). siRNA transfection was performed using Lipofectamine RNAiMAX according to the manufacturer's instructions (Invitrogen) with slight modifications. Briefly, 0.5×106 NRVMs were transfected in 2 ml of F-10 medium containing 500 μl of Opti-MEM (Invitrogen), 8 μl of Lipofectamine RNAiMAX and 100 nmol of siRNA.

Protocol tips

Primary neonatal rat ventricular myocyte (NRVM) cultures were prepared from 1–2-day-old rats using standard methods as previously described [10]. Cells were cultured in growth medium consisting of 10% Horse Serum (HS), 5% Fetal Bovine Serum (FBS) and 1% Penicillin/Streptomycin (PS) in F-10 Nutrient Mixture Media. The experiments were carried 36 h after cells were seeded. Pre-designed siRNA targeting rat p38 mRNA and an siRNA control were obtained from Invitrogen (siRNA p38 s135447 and siRNA control AM4611). siRNA transfection was performed using Lipofectamine RNAiMAX according to the manufacturer's instructions (Invitrogen) with slight modifications. Briefly, 0.5×106 NRVMs were transfected in 2 ml of F-10 medium containing 500 μl of Opti-MEM (Invitrogen), 8 μl of Lipofectamine RNAiMAX and 100 nmol of siRNA.

Publication protocol

Primary neonatal rat ventricular myocyte (NRVM) cultures were prepared from 1–2-day-old rats using standard methods as previously described [10]. Cells were cultured in growth medium consisting of 10% Horse Serum (HS), 5% Fetal Bovine Serum (FBS) and 1% Penicillin/Streptomycin (PS) in F-10 Nutrient Mixture Media. The experiments were carried 36 h after cells were seeded. Pre-designed siRNA targeting rat p38 mRNA and an siRNA control were obtained from Invitrogen (siRNA p38 s135447 and siRNA control AM4611). siRNA transfection was performed using Lipofectamine RNAiMAX according to the manufacturer's instructions (Invitrogen) with slight modifications. Briefly, 0.5×106 NRVMs were transfected in 2 ml of F-10 medium containing 500 μl of Opti-MEM (Invitrogen), 8 μl of Lipofectamine RNAiMAX and 100 nmol of siRNA.

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