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Cells were transfected with or without indicated plasmid DNA, siRNA constructs, or scrambled siRNA using lipofectamine 2000 according to the manufacturer's instructions. The transfected cells were allowed to grow in Complete medium for 24–72 h posttransfection before treatment with various stimuli as indicated. |
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Protocol tips |
Cells were transfected with or without indicated plasmid DNA, siRNA constructs, or scrambled siRNA using lipofectamine 2000 according to the manufacturer's instructions. The transfected cells were allowed to grow in Complete medium for 24–72 h posttransfection before treatment with various stimuli as indicated. |
Publication protocol
Cells were transfected with or without indicated plasmid DNA, siRNA constructs, or scrambled siRNA using lipofectamine 2000 according to the manufacturer's instructions. The transfected cells were allowed to grow in Complete medium for 24–72 h posttransfection before treatment with various stimuli as indicated.
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Manufacturer protocol
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