ON-TARGETplus Rat Smad3 (25631) siRNA - SMARTpool

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	The siRNAs specific for rat Smad2 and Smad3 (ON-TARGET plus SMARTpool, a mixture of four prevalidated siRNAs) and the matched negative control (nontarget control SMARTpool) were purchased from Thermo Scientific Dharmacon (Epsom, UK). The Smad2, Smad3, and negative control siRNAs were transfected twice on two consecutive days using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions

Protocol tips

The siRNAs specific for rat Smad2 and Smad3 (ON-TARGET plus SMARTpool, a mixture of four prevalidated siRNAs) and the matched negative control (nontarget control SMARTpool) were purchased from Thermo Scientific Dharmacon (Epsom, UK). The Smad2, Smad3, and negative control siRNAs were transfected twice on two consecutive days using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions

Publication protocol

The siRNAs specific for rat Smad2 and Smad3 (ON-TARGET plus SMARTpool, a mixture of four prevalidated siRNAs) and the matched negative control (nontarget control SMARTpool) were purchased from Thermo Scientific Dharmacon (Epsom, UK). The Smad2, Smad3, and negative control siRNAs were transfected twice on two consecutive days using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions

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Manufacturer protocol

Download the product protocol from Horizon Discovery Ltd. for ON-TARGETplus Rat Smad3 (25631) siRNA - SMARTpool below.

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