IL-4Rα shRNA (m) Lentiviral Particles

shRNA gene silencing Mouse - R221a IL4Rα

Experiment
shRNA gene silencing Mouse - R221a IL4Rα
Product
IL-4Rα shRNA (m) Lentiviral Particles from Santa Cruz Biotechnology
Manufacturer
Santa Cruz Biotechnology

Protocol tips

Protocol tips
PyVT-R221a cells were isolated from a polyoma middle T oncoprotein (PyMT) tumor as previously described [13]. The R221a line was authenticated by expression of the polyoma antigen, and the morphology and growth rates of both cell lines were consistently monitored. Cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 10% fetal bovine serum (FBS, Atlanta Biologicals) at 37°C with 5% CO2. Control non-target and specific murine IL4Ra-targeted shRNA lentiviral particles (Santa Cruz Biotechnologies) were used to infect R221a cells. Post-infection, shRNA-expressing cells were selected with puromycin dihydrochloride (Sigma). The percent of IL4Rα protein knockdown was calculated by densitometry in Adobe Photoshop® following western blot analysis.

Publication protocol

PyVT-R221a cells were isolated from a polyoma middle T oncoprotein (PyMT) tumor as previously described [13]. The R221a line was authenticated by expression of the polyoma antigen, and the morphology and growth rates of both cell lines were consistently monitored. Cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 10% fetal bovine serum (FBS, Atlanta Biologicals) at 37°C with 5% CO2. Control non-target and specific murine IL4Ra-targeted shRNA lentiviral particles (Santa Cruz Biotechnologies) were used to infect R221a cells. Post-infection, shRNA-expressing cells were selected with puromycin dihydrochloride (Sigma). The percent of IL4Rα protein knockdown was calculated by densitometry in Adobe Photoshop® following western blot analysis.

Full paper   Login or join for free to view the full paper.

Reviews

IL-4Rα shRNA (m) Lentiviral Particles from Santa Cruz Biotechnology has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: A.C.Burton United Kingdom

Multiple gene silencing using ShRNA

Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing shRNA gene silencing Mouse - R221a IL4Rα using IL-4Rα shRNA (m) Lentiviral Particles from Santa Cruz Biotechnology.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for IL-4Rα shRNA (m) Lentiviral Particles below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing shRNA gene silencing Mouse - R221a IL4Rα using IL-4Rα shRNA (m) Lentiviral Particles from Santa Cruz Biotechnology. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms