Individual: TRC Mouse Cdh1 shRNA

Protocol tips

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	The 4T1 (Murine mammary carcinoma cell line, ATCC® CRL-2539™) were cultured in PRMI-1640 (GibcoTM, Waltham, MA, USA) plus 10% FBS, 2mM L-glutamine (Biological Industries), and 1% Pen-Strep (Biological Industries). TRC cdh1 shRNA - TRCN0000042578 (GE Healthcare Dharmacon, Lafayette, CO, USA); pCMV-VSV-G (helper plasmid) and pHRCMV-8.2ΔR (packaging plasmid) were both kindly provided by Prof. Yardena Samuels (Weizmann Institute of Science). Mission pLKO.1-puro (Sigma-Aldrich) was used as a negative control vector, to create 4T1-shCon cells. Cells were transfected using Lipofectamine2000® (InvitrogenTM), according to the manufacturer’s instructions.

Protocol tips

The 4T1 (Murine mammary carcinoma cell line, ATCC® CRL-2539™) were cultured in PRMI-1640 (GibcoTM, Waltham, MA, USA) plus 10% FBS, 2mM L-glutamine (Biological Industries), and 1% Pen-Strep (Biological Industries). TRC cdh1 shRNA - TRCN0000042578 (GE Healthcare Dharmacon, Lafayette, CO, USA); pCMV-VSV-G (helper plasmid) and pHRCMV-8.2ΔR (packaging plasmid) were both kindly provided by Prof. Yardena Samuels (Weizmann Institute of Science). Mission pLKO.1-puro (Sigma-Aldrich) was used as a negative control vector, to create 4T1-shCon cells. Cells were transfected using Lipofectamine2000® (InvitrogenTM), according to the manufacturer’s instructions.

Publication protocol

The 4T1 (Murine mammary carcinoma cell line, ATCC® CRL-2539™) were cultured in PRMI-1640 (GibcoTM, Waltham, MA, USA) plus 10% FBS, 2mM L-glutamine (Biological Industries), and 1% Pen-Strep (Biological Industries). TRC cdh1 shRNA - TRCN0000042578 (GE Healthcare Dharmacon, Lafayette, CO, USA); pCMV-VSV-G (helper plasmid) and pHRCMV-8.2ΔR (packaging plasmid) were both kindly provided by Prof. Yardena Samuels (Weizmann Institute of Science). Mission pLKO.1-puro (Sigma-Aldrich) was used as a negative control vector, to create 4T1-shCon cells. Cells were transfected using Lipofectamine2000® (InvitrogenTM), according to the manufacturer’s instructions.

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