BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector

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	The adenovirus-based vector AdFoxm1siRNA that expresses Foxm1-specific siRNA was constructed with Invitrogen Block-it Adenoviral RNAi Expression System (Cat. No K4941-00 and V492-20), following the manufacturer’s instructions. A siRNA sequence 5′-GGA CCA CTT CCC TTA CTT T-3 from mouse Foxm1 cDNA was used to design a double-strand DNA that was recombinated into the adenovirus vector. The constructions of human FOXM1-expression adenovirus AdFOXM1 and control virus AdLacZ or AdGFP were described previously (34). The large-scale adenovirus purification and viral infections were carried out as previously described (35). For siRNA treatment, mOct4 siRNA (sc-36124) and control siRNA (sc-37007) were purchased from Santa Cruz. The siRNA transfection was performed according to the manufacturer’s instructions.

Protocol tips

The adenovirus-based vector AdFoxm1siRNA that expresses Foxm1-specific siRNA was constructed with Invitrogen Block-it Adenoviral RNAi Expression System (Cat. No K4941-00 and V492-20), following the manufacturer’s instructions. A siRNA sequence 5′-GGA CCA CTT CCC TTA CTT T-3 from mouse Foxm1 cDNA was used to design a double-strand DNA that was recombinated into the adenovirus vector. The constructions of human FOXM1-expression adenovirus AdFOXM1 and control virus AdLacZ or AdGFP were described previously (34). The large-scale adenovirus purification and viral infections were carried out as previously described (35). For siRNA treatment, mOct4 siRNA (sc-36124) and control siRNA (sc-37007) were purchased from Santa Cruz. The siRNA transfection was performed according to the manufacturer’s instructions.

Publication protocol

The adenovirus-based vector AdFoxm1siRNA that expresses Foxm1-specific siRNA was constructed with Invitrogen Block-it Adenoviral RNAi Expression System (Cat. No K4941-00 and V492-20), following the manufacturer’s instructions. A siRNA sequence 5′-GGA CCA CTT CCC TTA CTT T-3 from mouse Foxm1 cDNA was used to design a double-strand DNA that was recombinated into the adenovirus vector. The constructions of human FOXM1-expression adenovirus AdFOXM1 and control virus AdLacZ or AdGFP were described previously (34). The large-scale adenovirus purification and viral infections were carried out as previously described (35). For siRNA treatment, mOct4 siRNA (sc-36124) and control siRNA (sc-37007) were purchased from Santa Cruz. The siRNA transfection was performed according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector below.

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