LDH Cytotoxicity Detection Kit

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- Cells were treated with different concentrations of genistein (5–100 μM) for 24, 48 and 72 h before measuring toxicity.

Upstream tips
- Cells were treated with different concentrations of genistein (5–100 μM) for 24, 48 and 72 h before measuring toxicity.

Publication protocol

Cellular toxicity was measured in 3T3-L1 preadipocytes using MTT and LDH assays with various concentrations of genistein (5–100 μM) for 24, 48 and 72 h. To measure lactate dehydrogenase (LDH) release, 100 μl/well supernatant medium was transferred to the corresponding well of an optically clear 96-well flat-bottom microtiter plate and analyzed using an LDH cytotoxicity detection kit (Takara Bio, Inc., Otsu, Japan).

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