FITC anti-mouse CD326 (Ep-CAM) Antibody

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	The cells were prepared for use with FITC anti-mouse CD326 (epithelial cell adhesion molecule: EpCAM) antibodies (Biolegend, San Diego, CA, USA) and FITC Rat IgG2a.k Isotype Ctrl antibodies (Biolegend). The viability rate of the EpCAM-positive cells was measured based on the negative expression of 7-aminoactinomycin D (7-AAD) Staining Solution (BD Biosciences, Bedford, MA, USA). All samples were pre-incubated for 15 min (the light was shaded during this time). The cells were analyzed and sorted using a FACS Aria II cell sorter (BD Biosciences).

Protocol tips

The cells were prepared for use with FITC anti-mouse CD326 (epithelial cell adhesion molecule: EpCAM) antibodies (Biolegend, San Diego, CA, USA) and FITC Rat IgG2a.k Isotype Ctrl antibodies (Biolegend). The viability rate of the EpCAM-positive cells was measured based on the negative expression of 7-aminoactinomycin D (7-AAD) Staining Solution (BD Biosciences, Bedford, MA, USA). All samples were pre-incubated for 15 min (the light was shaded during this time). The cells were analyzed and sorted using a FACS Aria II cell sorter (BD Biosciences).

Publication protocol

The cells were prepared for use with FITC anti-mouse CD326 (epithelial cell adhesion molecule: EpCAM) antibodies (Biolegend, San Diego, CA, USA) and FITC Rat IgG2a.k Isotype Ctrl antibodies (Biolegend). The viability rate of the EpCAM-positive cells was measured based on the negative expression of 7-aminoactinomycin D (7-AAD) Staining Solution (BD Biosciences, Bedford, MA, USA). All samples were pre-incubated for 15 min (the light was shaded during this time). The cells were analyzed and sorted using a FACS Aria II cell sorter (BD Biosciences).

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