In Situ Cell Death Detection Kit, Fluorescein

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were fixed with 4% paraformaldehyde for 20 min. - Permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice	- When Nonspecific labelling is present, reduce concentration of TdT. - When high background is present reduce concentration of labelling mix by 50%

Protocol tips
- Cells were fixed with 4% paraformaldehyde for 20 min. - Permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice
Downstream tips
- When Nonspecific labelling is present, reduce concentration of TdT. - When high background is present reduce concentration of labelling mix by 50%

Publication protocol

Sk-mel-2 cells mounted on glass slides were fixed with 4% paraformaldehyde for 20 min, and permeabilized with PBS containing 0.1% Triton X-100 and 0.1% glycine for 2 min on ice. The TUNEL staining was performed using the In Situ Cell Death Detection Kit, Fluorescein (Roche Diagnostics, Germany). Cells were co-stained with 4′6′-diamidino-2-phenylindole (DAPI) to visualize nuclei. The images were taken using a FSX100 all-in-one microscope (Olympus Corporation, Japan).

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

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