CD137 (4-1BB) Monoclonal Antibody (17B5), PE, eBioscience™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The flow cytometry was performed to observe the CD137 level of membrane.	Cells were digested and washed with PBS. Anti-CD137-PE (eBioscience, USA) was diluted according to the protocol and was added to 100 μl cell suspension for 30 min at 4°C. After the incubation, the cells were washed one time and then analyzed by flow cytometry (BD Canto).

Upstream tips
The flow cytometry was performed to observe the CD137 level of membrane.
Protocol tips
Cells were digested and washed with PBS. Anti-CD137-PE (eBioscience, USA) was diluted according to the protocol and was added to 100 μl cell suspension for 30 min at 4°C. After the incubation, the cells were washed one time and then analyzed by flow cytometry (BD Canto).

Publication protocol

The flow cytometry was performed to observe the CD137 level of membrane, which further validates the results of western blot. Cells were digested and washed with PBS. Anti-CD137-PE (eBioscience, USA) was diluted according to the protocol and was added to 100 μl cell suspension for 30 min at 4°C. After the incubation, the cells were washed one time and then analyzed by flow cytometry (BD Canto).

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Manufacturer protocol

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