CD140a (PDGFRA) Monoclonal Antibody (APA5), PE, eBioscience™

Protocol tips

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	Mouse lung fibroblasts were plated onto 6-well plates (1×105/well) and incubated in RPMI1640 medium supplemented with 10% FBS at 37°C under 5% CO2 in humidified air. Cells (5×105) were collected and incubated for 30 minutes on ice with PE anti-mouse CD140a (PDGFR-α) antibody	Stained cells were analyzed by flow cytometry using BD LSRFortessa for acquisition and BD FACSDiva software 6.0 (BD Biosciences, San Jose, CA) for analyses

Protocol tips
Mouse lung fibroblasts were plated onto 6-well plates (1×105/well) and incubated in RPMI1640 medium supplemented with 10% FBS at 37°C under 5% CO2 in humidified air. Cells (5×105) were collected and incubated for 30 minutes on ice with PE anti-mouse CD140a (PDGFR-α) antibody
Downstream tips
Stained cells were analyzed by flow cytometry using BD LSRFortessa for acquisition and BD FACSDiva software 6.0 (BD Biosciences, San Jose, CA) for analyses

Publication protocol

Flow cytometry was performed for the detection of PDGFR-α and -β in mouse lung fibroblasts and mouse alveolar epithelial cells. Mouse lung fibroblasts were plated onto 6-well plates (1×105/well) and incubated in RPMI1640 medium supplemented with 10% FBS at 37°C under 5% CO2 in humidified air. Cells (5×105) were collected and incubated for 30 minutes on ice with PE anti-mouse CD140a (PDGFR-α) antibody, PE anti-mouse CD140b (PDGFR-β) antibody and purified RAT IgG (Invitrogen). Mouse alveolar epithelial cells were isolated using MACS as described above. Cells were incubated with a FITC anti-mouse CD326 (EpCAM) antibody, PE anti-mouse CD140a (PDGFR-α) antibody, and PE anti-mouse CD140b (PDGFR-β) antibody (Biolegend). Stained cells were analyzed by flow cytometry using BD LSRFortessa for acquisition and BD FACSDiva software 6.0 (BD Biosciences, San Jose, CA) for analyses

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