PE anti-mouse CD146 Antibody

Protocol tips

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	Adherent endothelial cells were detached from culture flasks with trypsin/EDTA (Life Technologies), washed and resuspended in PBS (Gibco Life Technologies) containing 1% FCS. Cells (106 cells/ml) were then incubated with respective FITC-, PE- or APC-conjugated monoclonal antibodies for 20 min at 4°C. Fluorescence antibody-labeled cells were then washed twice in cold PBS with 1% FCS and analyzed using a flow cytometer (BD Bioscience; San Jose, CA, USA).

Protocol tips

Adherent endothelial cells were detached from culture flasks with trypsin/EDTA (Life Technologies), washed and resuspended in PBS (Gibco Life Technologies) containing 1% FCS. Cells (106 cells/ml) were then incubated with respective FITC-, PE- or APC-conjugated monoclonal antibodies for 20 min at 4°C. Fluorescence antibody-labeled cells were then washed twice in cold PBS with 1% FCS and analyzed using a flow cytometer (BD Bioscience; San Jose, CA, USA).

Publication protocol

Adherent endothelial cells were detached from culture flasks with trypsin/EDTA (Life Technologies), washed and resuspended in PBS (Gibco Life Technologies) containing 1% FCS. Cells (106 cells/ml) were then incubated with respective FITC-, PE- or APC-conjugated monoclonal antibodies for 20 min at 4°C. Fluorescence antibody-labeled cells were then washed twice in cold PBS with 1% FCS and analyzed using a flow cytometer (BD Bioscience; San Jose, CA, USA).

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