FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

TUNEL assay cell type - 3T3 L1 mouse adipose tissue

Experiment
TUNEL assay cell type - 3T3 L1 mouse adipose tissue
Product
FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme from Millipore
Manufacturer
Millipore

Protocol tips

Protocol tips
- Cells in 4% formaldehyde/PBS at a cell density of 1x10^6 /ml

- Negative controls were performed by substituting Tris buffered saline (TBS) for the TdT enzyme.

Publication protocol

Cell cultures were fixed with 4% paraformaldehyde, and in situ detection of cells with DNA strand breaks was performed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method using a Fluorescein FragEL™ DNA Fragmentation Detection Kit (Calbiochem) according to the manufacturer's instructions. Negative controls were performed by substituting Tris buffered saline (TBS) for the TdT enzyme. Cells labeled with the Fluorescein-FragEL DNA fragmentation detection kit (Cat No. QIA39) were analyzed with a BD FACSCalibur flow cytometer (BD Biosciences).

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Discussion

Discussion

2 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - 3T3 L1 mouse adipose tissue using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme from Millipore.

Paper title
Inhibition of Adipogenesis and Induction of Apoptosis and Lipolysis by Stem Bromelain in 3T3-L1 Adipocytes
Full paper
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Manufacturer protocol

Download the product protocol from Millipore for FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme below.

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