FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

Protocol tips

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	- Cells in 4% formaldehyde/PBS at a cell density of 1x10^6 /ml - Negative controls were performed by substituting Tris buffered saline (TBS) for the TdT enzyme.

Protocol tips
- Cells in 4% formaldehyde/PBS at a cell density of 1x10^6 /ml - Negative controls were performed by substituting Tris buffered saline (TBS) for the TdT enzyme.

Publication protocol

Cell cultures were fixed with 4% paraformaldehyde, and in situ detection of cells with DNA strand breaks was performed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method using a Fluorescein FragEL™ DNA Fragmentation Detection Kit (Calbiochem) according to the manufacturer's instructions. Negative controls were performed by substituting Tris buffered saline (TBS) for the TdT enzyme. Cells labeled with the Fluorescein-FragEL DNA fragmentation detection kit (Cat No. QIA39) were analyzed with a BD FACSCalibur flow cytometer (BD Biosciences).

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Manufacturer protocol

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