DeadEnd™ Colorimetric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Downstream tips
- When there is a loss of tissue from slides, decrease Proteinase K incubation time.

Publication protocol

Apoptosis in adipocytes was observed and detected by various methods, including caspase 3/7 activation analysis, TUNEL assay, and flow cytometry. Caspase 3/7 activity was quantitated by using a Caspase-Glo® 3/7 Assay Kit (Promega, CA, USA) according to the manufacturer's instructions. A DeadEnd™ Fluorometric TUNEL System (Promega) was used to observe and measure the adipocyte apoptosis. The number of apoptotic adipocytes was quantitated by flow cytometry assay (Gallios, Beckman Coulter, USA) with the Annexin V/PI double-staining method according to the manufacturer's instructions. (The Annexin V/PI apoptosis assay kit was purchased from Life Technologies, USA).

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

Download manufacturer protocol

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