PE anti-mouse CD138 (Syndecan-1) Antibody

Protocol tips

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	Cells were surface-stained, then fixed/permeabilized (Fix-Perm buffer, eBioscience) before intracellular staining.

Protocol tips
Cells were surface-stained, then fixed/permeabilized (Fix-Perm buffer, eBioscience) before intracellular staining.

Publication protocol

Flow cytometry was performed as described (21) using anti-mouse CD16/32 (Fc Block; BD Biosciences) before staining with primary antibody or isotype controls. Cells were surface-stained, then fixed/permeabilized (Fix-Perm buffer, eBioscience) before intracellular staining. Antibodies are listed in Table ​Table2.2. Uptake of low-density lipoproteins was assessed by incubating PEC with BODIPY-labeled LDL (10 µg/ml, Invitrogen) (16). Data were acquired and analyzed as above. CD11b+Ly6Chi LyG- and CD11b+CD138+ Ly6G-cells were sorted using a FACSaria cell sorter and 40,000 cells/subset were lysed immediately for RNA extraction.

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