In Situ Cell Death Detection Kit, Fluorescein

TUNEL assay cell type - 3T3 L1 mouse adipose tissue

Experiment
TUNEL assay cell type - 3T3 L1 mouse adipose tissue
Product
In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Fix cells in n 4% (w/v) paraformaldehyde for 1 h at room temperature

Permeabilize cells with 0.5% (v/v) Triton X-100 in PBS for 1 h at 37 °C.

Add Tunel mixture and incubate for 1 h at 37 °C in dark.

Publication protocol

TUNEL assay was used to measure cell apoptosis. Cells were seeded on glass coverslips in six-well dishes, and at 48 h after differentiation, staining of fragmented DNA from apoptotic cells was carried out using an in situ Cell Death Detection Kit Fluorescein (Roche). Briefly, cells were rinsed with PBS followed by fixation in 4% (w/v) paraformaldehyde for 1 h at room temperature. Fixed cells were permeabilized by incubation with 0.5% (v/v) Triton X-100 in PBS for 1 h at 37 °C. The cells were rinsed again with PBS and incubated with 50 μl TUNEL reaction mixture per sample for 1 h at 37 °C. Following washes with PBS, samples were mounted on glass slides with Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA) and examined by inverted fluorescence confocal microscopy.

Full paper   Login or join for free to view the full paper.

Reviews

In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

2 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - 3T3 L1 mouse adipose tissue using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich.

Paper title
G0/G1 switch gene 2 has a critical role in adipocyte differentiation
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for In Situ Cell Death Detection Kit, Fluorescein below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - 3T3 L1 mouse adipose tissue using In Situ Cell Death Detection Kit, Fluorescein from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms