VasoTACS™ In Situ Apoptosis Detection Kit

TUNEL assay cell type - Mouse endothelial cells

Experiment

TUNEL assay cell type - Mouse endothelial cells

Product

VasoTACS™ In Situ Apoptosis Detection Kit from Bio-Techne

Manufacturer

Bio-Techne

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Treat with Proteinase K Solution for 15-30 minutes or Cytonin for 30 minutes. Treat with Labeling Reaction Mix and incubate for 60 minutes at 37˚C in a humidity chamber. Treat with TACS Blue LabelTM (page 5), and incubate for 2-7 minute.	If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC).

Protocol tips
Treat with Proteinase K Solution for 15-30 minutes or Cytonin for 30 minutes. Treat with Labeling Reaction Mix and incubate for 60 minutes at 37˚C in a humidity chamber. Treat with TACS Blue LabelTM (page 5), and incubate for 2-7 minute.
Downstream tips
If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC).

Publication protocol

Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labelling (TUNEL) assays were performed on paraffin-embedded sections prepared from E13.5 using EC-specific VasoTACS in situ apoptosis detection kit (Trevigen).36,37 For imaging and quantification, 20 microscopic fields were selected randomly, photographed with Zeiss Apotome microscope acquired using AxioVision software at room temperature. TUNEL-positive ECs were quantified in both MT and their WT littermates. Experiments were repeated at least three times.

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Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Mouse endothelial cells using VasoTACS™ In Situ Apoptosis Detection Kit from Bio-Techne.

Paper title

Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development

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Manufacturer protocol

Download the product protocol from Bio-Techne for VasoTACS™ In Situ Apoptosis Detection Kit below.

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