|Treat with Proteinase K Solution for 15-30 minutes or Cytonin for 30 minutes.
Treat with Labeling Reaction Mix and incubate for 60
minutes at 37˚C in a humidity chamber.
Treat with TACS Blue LabelTM (page 5), and incubate for 2-7
|If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to 60 minutes) or Cytonin™ (up to overnight at 4 oC).|
VasoTACS™ In Situ Apoptosis Detection Kit from Bio-Techne has not yet been reviewed for this experiment
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4 years ago
Author: Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Mouse endothelial cells using VasoTACS™ In Situ Apoptosis Detection Kit from Bio-Techne.
Download the product protocol from Bio-Techne for VasoTACS™ In Situ Apoptosis Detection Kit below.Download manufacturer protocol
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