Purified Rat Anti-Mouse IFN-γ

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cells were stained with fixable viability dye eF780 or eF506 (eBioscience). ). All cells were fixed and permeabilized by the FOXP3 Fixation/Permeabilization Concentrate and Diluent kit (eBioscience).

Protocol tips
Cells were stained with fixable viability dye eF780 or eF506 (eBioscience). ). All cells were fixed and permeabilized by the FOXP3 Fixation/Permeabilization Concentrate and Diluent kit (eBioscience).

Publication protocol

Spleens, lymph nodes, lungs, and livers were harvested for IL-17 analysis on d28, d42, and d56, and single cell suspensions were made. The cells were stimulated with Cell Stimulation Cocktail plus Protein Transport Inhibitors (eBioscience, 00-4975-93) for 5 hours at 37°C in complete-RPMI. Cells were stained with fixable viability dye eF780 or eF506 (eBioscience), anti-CD4 (eBioscience, clone GK1.5), anti–IFN-γ (BD Biosciences, clone XMG1.2), anti–IL-17A (eBioscience, clone eBio17B7), anti–IL-21 (eBioscience, clone FFA21), anti-RORγt (eBioscience, clone B2D), anti-CCR5 (eBioscience, clone HM-CCR5 [7A4]), anti-CD146 (BD Biosciences, clone ME-9F1), and anti-CD45.2 (BD Biosciences, clone 104). All cells were fixed and permeabilized by the FOXP3 Fixation/Permeabilization Concentrate and Diluent kit (eBioscience).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Mouse - IFN-γ using Purified Rat Anti-Mouse IFN-γ from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for Purified Rat Anti-Mouse IFN-γ below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Mouse - IFN-γ using Purified Rat Anti-Mouse IFN-γ from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.