Purified anti-mouse CD68 Antibody

Protocol tips

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	Marrow was flushed from the femur of mice immediately after sacrifice in FACS buffer (PBS with 2%FBS, 0.5 mM EDTA). Each stain included 106 cells with anti-mouse F4/80 (Biolegend, or Abcam, A3-1) or anti-mouse CD68 (Biolegend, FA-11). Isotype controls were used to confirm specificity. Flow cytometry was performed using a FACS AriaIII (BD Biosciences).

Protocol tips
Marrow was flushed from the femur of mice immediately after sacrifice in FACS buffer (PBS with 2%FBS, 0.5 mM EDTA). Each stain included 106 cells with anti-mouse F4/80 (Biolegend, or Abcam, A3-1) or anti-mouse CD68 (Biolegend, FA-11). Isotype controls were used to confirm specificity. Flow cytometry was performed using a FACS AriaIII (BD Biosciences).

Publication protocol

Marrow was flushed from the femur of mice immediately after sacrifice in FACS buffer (PBS with 2%FBS, 0.5 mM EDTA). Each stain included 106 cells with anti-mouse F4/80 (Biolegend, or Abcam, A3-1) or anti-mouse CD68 (Biolegend, FA-11). Isotype controls were used to confirm specificity. Flow cytometry was performed using a FACS AriaIII (BD Biosciences).

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