CD206 antibody | MR5D3

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	An isotype control antibody was used as a negative control.	The experiments were performed using a FACS Diva Version 6.1.2 automated cell analyzer (BD FACS Canto II).

Protocol tips
An isotype control antibody was used as a negative control.
Downstream tips
The experiments were performed using a FACS Diva Version 6.1.2 automated cell analyzer (BD FACS Canto II).

Publication protocol

"Isolation and separation of the stromal vascular fraction (SVF) and subsequent flow cytometry were performed as previously described8,12,35. After the exclusion of dead cells by gating with 7-amino-actinomyciin D (7AAD), live cells in the SVF of ingWAT were selected for further analysis. First, CD31/CD45+ (endothelial/hematopoietic) cells were negatively selected (1:100), followed by the positive selection of CD137+ cells (1:80). For CD206+ M2-like macrophages, after removing debris, live cells were gated for CD45+ (hematopoietic), and then CD45+ cells were gated for the F4/80+CD206+ double positive population, which was characterized as consisting of M2-like macrophages. An isotype control antibody was used as a negative control. The experiments were performed using a FACS Diva Version 6.1.2 automated cell analyzer (BD FACS Canto II).

Statistical analysis
Data are expressed as the mean ± SEM. Statistical significance was determined by unpaired Student’s t tests (for comparisons of two groups or two experimental conditions). Differences were considered statistically significant at values of *p < 0.05 or **p < 0.01."

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Mouse - CD206 using CD206 antibody | MR5D3 from Bio-Rad Laboratories.

Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for CD206 antibody | MR5D3 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Mouse - CD206 using CD206 antibody | MR5D3 from Bio-Rad Laboratories. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.