Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody

Protocol tips

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	To obtain single-cell suspensions, tumor tissues were digested by 1 mg/ml collagenase IV (Sigma-Aldrich) and 0.2 mg/ml DNase I (Sigma-Aldrich) for 45 minutes at 37°C. Cells were blocked with anti-FcR (clone 2.4G2; Bio-Xcell) and then stained with antibodies against PD-L1, PD-1, CD11b, Gr1, F4/80, CD11c, CD8, CD4, Foxp3, and CD45 (BioLegend). For apoptosis assays, MDSCs were harvested, blockaded with anti-FcR, and stained with antibodies against Ly6C, CD11b, and annexin V. Samples were collected on a FACSCalibur Flow Cytometer (BD), and data were analyzed using FlowJo software (Tree Star Inc.).

Protocol tips

To obtain single-cell suspensions, tumor tissues were digested by 1 mg/ml collagenase IV (Sigma-Aldrich) and 0.2 mg/ml DNase I (Sigma-Aldrich) for 45 minutes at 37°C. Cells were blocked with anti-FcR (clone 2.4G2; Bio-Xcell) and then stained with antibodies against PD-L1, PD-1, CD11b, Gr1, F4/80, CD11c, CD8, CD4, Foxp3, and CD45 (BioLegend). For apoptosis assays, MDSCs were harvested, blockaded with anti-FcR, and stained with antibodies against Ly6C, CD11b, and annexin V. Samples were collected on a FACSCalibur Flow Cytometer (BD), and data were analyzed using FlowJo software (Tree Star Inc.).

Publication protocol

To obtain single-cell suspensions, tumor tissues were digested by 1 mg/ml collagenase IV (Sigma-Aldrich) and 0.2 mg/ml DNase I (Sigma-Aldrich) for 45 minutes at 37°C. Cells were blocked with anti-FcR (clone 2.4G2; Bio-Xcell) and then stained with antibodies against PD-L1, PD-1, CD11b, Gr1, F4/80, CD11c, CD8, CD4, Foxp3, and CD45 (BioLegend). For apoptosis assays, MDSCs were harvested, blockaded with anti-FcR, and stained with antibodies against Ly6C, CD11b, and annexin V. Samples were collected on a FACSCalibur Flow Cytometer (BD), and data were analyzed using FlowJo software (Tree Star Inc.).

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Manufacturer protocol

Download the product protocol from BioLegend for Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody below.

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