APC anti-mouse CD117 (c-kit) Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For c-kit+/SSEA1− cell isolation, cells were detached using HyQTase (Thermo Fisher Scientific), blocked with Fc (BioLegend, San Diego, CA, USA) for 15 min, and then incubated with the antibody at 4 °C for 30 min. Rinsing with staining buffer (eBioscience, San Diego, CA, USA).	The cells were purified using a FACSAria Flow Cytometer (BD Bioscience). The purified cells were passaged five times before differentiation assays and cell transplantation.

Protocol tips
For c-kit+/SSEA1− cell isolation, cells were detached using HyQTase (Thermo Fisher Scientific), blocked with Fc (BioLegend, San Diego, CA, USA) for 15 min, and then incubated with the antibody at 4 °C for 30 min. Rinsing with staining buffer (eBioscience, San Diego, CA, USA).
Downstream tips
The cells were purified using a FACSAria Flow Cytometer (BD Bioscience). The purified cells were passaged five times before differentiation assays and cell transplantation.

Publication protocol

For c-kit+/SSEA1− cell isolation, cells were detached using HyQTase (Thermo Fisher Scientific), blocked with Fc (BioLegend, San Diego, CA, USA) for 15 min, and then incubated with anti-mouse c-kit antibody conjugated with APC (BioLegend) and anti-mouse SSEA1 antibody conjugated with FITC (BD Biosciences) at 4 °C for 30 min. After rinsing with staining buffer (eBioscience, San Diego, CA, USA), the cells were purified for the c-kit-positive, SSEA1-negative population using a FACSAria Flow Cytometer (BD Bioscience). The purified cells were passaged five times before differentiation assays and cell transplantation.

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Manufacturer protocol

Download the product protocol from BioLegend for APC anti-mouse CD117 (c-kit) Antibody below.

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