Purified anti-mouse TCR γ/δ Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Liver tissues were processed by enzymatic digestion with 2mg/mL type IV collagenase (Worthington, NY). In some experiments, cells were stimulated with 50ng/mL Phorbol 12-Myristate 13-Acetate (PMA; LC Labs, USA) and 1μg/mL Ionomycin (Enzo Life sciences, USA) for 4 hours in the presence of Golgi Stop/Golgi Plug (BD Biosciences).		Cells were analyzed using BD LSRII and FlowJo9.6.4 (TreeStar, Oregon, USA).

Upstream tips
Liver tissues were processed by enzymatic digestion with 2mg/mL type IV collagenase (Worthington, NY). In some experiments, cells were stimulated with 50ng/mL Phorbol 12-Myristate 13-Acetate (PMA; LC Labs, USA) and 1μg/mL Ionomycin (Enzo Life sciences, USA) for 4 hours in the presence of Golgi Stop/Golgi Plug (BD Biosciences).
Downstream tips
Cells were analyzed using BD LSRII and FlowJo9.6.4 (TreeStar, Oregon, USA).

Publication protocol

Liver tissues were processed by enzymatic digestion with 2mg/mL type IV collagenase (Worthington, NY). In some experiments, cells were stimulated with 50ng/mL Phorbol 12-Myristate 13-Acetate (PMA; LC Labs, USA) and 1μg/mL Ionomycin (Enzo Life sciences, USA) for 4 hours in the presence of Golgi Stop/Golgi Plug (BD Biosciences). The following antibodies were used: anti-CD3 (17A2), CD8 (53–6.7), CD4 (RM4-5), CD11c (N418), GR1 (RB6-8C5), I/A-I/E (M5/114.15.2), TCRβ (H57-597), CD45.1 (A20), IFNγ (XMG1.2) (Tonbo Biosciences, USA). Anti- Ly6C (HK1.4), anti-CD11b (M1/70), TCRγδ (GL3), goat anti-hamster IgG (Poly4055), anti-TCRVγ1.1+1.2 (4B2.9), TCRVγ2 (UC3-10A6), TCRVγ3 (536), and anti-IL-17A (TC11-18H10.1) were purchased from Biolegend (USA). Anti-mouse TCRVγ4 (49.2) and TCRVγ7 (F2.64) were the kind gift from Dr. Pablo Pereira-Esteva (Pasteur Institute, France). Cells were analyzed using BD LSRII and FlowJo9.6.4 (TreeStar, Oregon, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Mouse - TCRγ/δ using Purified anti-mouse TCR γ/δ Antibody from BioLegend.

Manufacturer protocol

Download the product protocol from BioLegend for Purified anti-mouse TCR γ/δ Antibody below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Mouse - TCRγ/δ using Purified anti-mouse TCR γ/δ Antibody from BioLegend. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.