FITC anti-mouse CD95 (Fas) Antibody

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	Cell suspensions were incubated with purified anti‐CD16/32 antibody (BioLegend) for 15 minutes at 4°C, and then stained for 20 minutes at 4°C with a cocktail of fluorochrome‐conjugated antibodies against cell surface markers.

Protocol tips
Cell suspensions were incubated with purified anti‐CD16/32 antibody (BioLegend) for 15 minutes at 4°C, and then stained for 20 minutes at 4°C with a cocktail of fluorochrome‐conjugated antibodies against cell surface markers.

Publication protocol

Cell suspensions were incubated with purified anti‐CD16/32 antibody (BioLegend) for 15 minutes at 4°C, and then stained for 20 minutes at 4°C with a cocktail of fluorochrome‐conjugated antibodies against cell surface markers. The procedure for measurement of intracellular cytokines was performed as previously described (23), using the following monoclonal antibodies (mAb) (all from BioLegend): for mouse cells, Pacific Blue (PB)–conjugated CD3 (17A2), APC‐conjugated NK1.1 (PK136), fluorescein isothiocyanate (FITC)–conjugated CD19 (6D5), PE–Cy7–conjugated CD138 (281‐2), FITC‐conjugated Fas (15A7), PerCP–Cy5.5–conjugated GL7 (GL7), PerCP–Cy5.5–conjugated CD4 (GK1.5), APC–Cy7–conjugated CD8a (53‐6.7), PerCP–Cy5.5–conjugated CD8b (YTS156.7.7), PE–Cy7–conjugated CD11b (M1/70), PE‐conjugated F4/80 (BM8), anti‐CD16/32 (93), FITC‐conjugated CD69 (H1.2F3), PE–Cy7–conjugated CD103 (2E7), PE‐conjugated CXCR3 (CXCR3‐173), PE‐conjugated CXCR6 (SA051D1), APC‐conjugated CD49a (HMa1), and PE‐conjugated IFNγ (XMG1.2); for human cells, APC–Cy7–conjugated CD45 (HI30), PE‐conjugated CD56 (5.1H11), PB‐conjugated CD3 (SK7), PerCP–Cy5.5–conjugated CD8 (HIT8α), FITC‐conjugated CD69 (FN50), and APC‐conjugated CD103 (Ber‐ACT8). In addition, mAb against mouse V500‐conjugated CD45.2 (104), mouse V500‐conjugated B220 (RA3‐6B2), and human V500‐conjugated CD4 (RPA‐T4) were purchased from BD Biosciences.

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Manufacturer protocol

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