OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol, we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media.	- Due to light-induced auto-oxidation, DCFH-DA solutions at any concentration must be protected from light.

Protocol tips
- 1X DCFH-DA/media solution contains 5% methanol. For cells that are sensitive to methanol, we recommend instead preparing a 0.1X (100 µM) solution of DCFH-DA in cell culture media.
Downstream tips
- Due to light-induced auto-oxidation, DCFH-DA solutions at any concentration must be protected from light.

Publication protocol

Intracellular ROS generation was measured with an OxiSelect ROS Assay Kit (CELL BIOLABS, San Diego, CA) according to the manufacturer's instructions. The cell membrane-permeable fluorescent dye 2’,7’-dichlorofluorescein diacetate (DCFH-DA) was added to cells. DCFH-DA is converted to the impermeable nonfluorescent compound DCFH by intracellular esterases. Highly fluorescent DCF is produced by oxidation of DCFH by ROS. The fluorescence intensity of DCF inside the cells was measured using an EnVision plate reader. Triplicate wells were analyzed in each assay.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.