OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

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		- ROS assay were read at 480/530 nm

Downstream tips
- ROS assay were read at 480/530 nm

Publication protocol

SH-SY5Y cells (2 × 105) were seeded into 12-well culture plates, treated with 1000 μg/ml EBN and the equivalence 5 μg/ml LF and 10 μg/ml OVF, then followed by 250 μM H2O2 for 2 h. Protocols for the ELISA tests were based on manufacturer instructions. For SOD assay, absorbance of samples were finally read at 490 nm on the Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA), while fluorescence for ROS assay were read at 480/530 nm by Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

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