APC anti-human CD326 (EpCAM) Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		All stained cell suspensions were detected or sorted using an AccuriC6 cytometer (BD Biosciences) and analyzed by FlowJo 7.6.1.

Downstream tips
All stained cell suspensions were detected or sorted using an AccuriC6 cytometer (BD Biosciences) and analyzed by FlowJo 7.6.1.

Publication protocol

"Parent cells and enriched cells were incubated with LGR5 (#ab75735; Abcam) and an isotype control (#ab171870; Abcam) and subsequently with secondary antibodies (Alexa Fluor 488) for 30 min at room temperature and washed three times in phosphate buffered saline (PBS) after each reaction to detect the proportion of LGR5 expression. Using the method described above, LGR5+ and LGR5− glioma cells were sorted from parent cells by FACS.

To explore the proportion of reported stem cell markers in both LGR5+ and LGR5− glioma cells, the cells were incubated with APC anti-human CD133 antibody (#130–090-826; Miltenyi), APC anti-human CD44 antibody (#17–0441-82; eBioscience), APC anti-human CD24 antibody (#311118; BioLegend), APC anti-human CD90 antibody (#328114; BioLegend) or APC anti-human EpCAM (#328208; BioLegend) antibody for 30 min at room temperature. APC anti-human IgG (#409306; BioLegend) was used as an isotype control for these markers. All stained cell suspensions were detected or sorted using an AccuriC6 cytometer (BD Biosciences) and analyzed by FlowJo 7.6.1."

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Flow cytometry Anti-bodies Human - CD326/EpCAM using APC anti-human CD326 (EpCAM) Antibody from BioLegend.

Manufacturer protocol

Download the product protocol from BioLegend for APC anti-human CD326 (EpCAM) Antibody below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Flow cytometry Anti-bodies Human - CD326/EpCAM using APC anti-human CD326 (EpCAM) Antibody from BioLegend. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.