CD326 (EpCAM) Antibody, anti-human, PE, REAfinity™

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C.	Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences).

Protocol tips
Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C.
Downstream tips
Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences).

Publication protocol

Prostate cells were suspended in PBS, 2 mM EDTA,0.5%BSA and stained with antibody for 15 minutes at 4°C. Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). Live single cells were gated based on scatter properties and analyzed for their surface marker expression. Cells were sorted and collected at 4°C using 100um nozzle and 23psi. Antibodies used for FACS include Epcam-PE (Miltenyi Biotech), CD44-FITC (ebioscience), and CD49f-APC (BioLegend).

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Manufacturer protocol

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