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Fluorescence-activated cell sorting (FACS) analysis was performed using a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ). The following antibodies were used to classify the cells: anti-CD3-PE-Cy5, anti-CD14-FITC, anti-HLA-DE-PE, anti-B7-H1-PE and anti-B7-H4-PE (BD PharMingen, Franklin Lakes, NJ). |
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Protocol tips |
Fluorescence-activated cell sorting (FACS) analysis was performed using a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ). The following antibodies were used to classify the cells: anti-CD3-PE-Cy5, anti-CD14-FITC, anti-HLA-DE-PE, anti-B7-H1-PE and anti-B7-H4-PE (BD PharMingen, Franklin Lakes, NJ). |
Publication protocol
Fluorescence-activated cell sorting (FACS) analysis was performed using a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ). The following antibodies were used to classify the cells: anti-CD3-PE-Cy5, anti-CD14-FITC, anti-HLA-DE-PE, anti-B7-H1-PE and anti-B7-H4-PE (BD PharMingen, Franklin Lakes, NJ).
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