ROS-Glo™ H2O2 Assay

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	- The cells were incubated with test compounds and H2O2 substrate solution for 6 h

Protocol tips
- The cells were incubated with test compounds and H2O2 substrate solution for 6 h

Publication protocol

The CellTiter 96 aqueous one solution cell proliferation assay (Promega Biosciences) was used for cell viability assessments. According to the manufacturer’s instructions, cells were incubated with CellTiter 96 aqueous one solution reagent for 3 h. The absorbance of 450 nm was analyzed using an iMark microplate absorbance reader (Bio-Rad). ROS-Glo H2O2 assay (Promega Biosciences) was used for an ROS assay. The cells were incubated with test compounds and H2O2 substrate solution for 6 h and then ROS-Glo detection solution was added. The luminescence was observed using Fluoroskan Ascent FL.

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Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

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