ROS-Glo™ H2O2 Assay

ROS assay cell type - SH-SY5Y human neuroblastoma

Experiment
ROS assay cell type - SH-SY5Y human neuroblastoma
Product
ROS-Glo™ H2O2 Assay from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
- The cells were incubated with test compounds and H2O2 substrate solution for 6 h

Publication protocol

The CellTiter 96 aqueous one solution cell proliferation assay (Promega Biosciences) was used for cell viability assessments. According to the manufacturer’s instructions, cells were incubated with CellTiter 96 aqueous one solution reagent for 3 h. The absorbance of 450 nm was analyzed using an iMark microplate absorbance reader (Bio-Rad). ROS-Glo H2O2 assay (Promega Biosciences) was used for an ROS assay. The cells were incubated with test compounds and H2O2 substrate solution for 6 h and then ROS-Glo detection solution was added. The luminescence was observed using Fluoroskan Ascent FL.

Full paper   Login or join for free to view the full paper.

Reviews

ROS-Glo™ H2O2 Assay from Promega has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - SH-SY5Y human neuroblastoma using ROS-Glo™ H2O2 Assay from Promega.

Paper title
NRF2 Regulates Expression under Oxidative Stress Conditions
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Promega for ROS-Glo™ H2O2 Assay below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - SH-SY5Y human neuroblastoma using ROS-Glo™ H2O2 Assay from Promega. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms