Nectin 1 Monoclonal Antibody (CK8)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	1:100 for anti–nectin-1 antibody (Zymed)

Protocol tips
1:100 for anti–nectin-1 antibody (Zymed)

Publication protocol

Cell surface expression of receptors was investigated through the use of flow cytometry. Fluorescence-activated cell sorter (FACS) analysis was performed on HCjE cells using the same primary and secondary antibodies used with immunocytochemistry experiments. The cells were treated with the antibodies, as described previously.9 Controls were used with HCjE treated with secondary antibody alone. Antibodies were administered at appropriate dilutions: 1:20 for anti–HVEM antibody, 1:100 for anti–nectin-1 antibody (Zymed), and 1:10 for HS4C3 antibody.

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Manufacturer protocol

Download the product protocol from eBioscience for Nectin 1 Monoclonal Antibody (CK8) below.

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