OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 50,000 cells/well were seeded	- Cells werre treated with α-TOS, NTα-TOS-NPs, or T-α-TOS-NPs for 12 h at 37 °C in 5% CO2. - RIPA cell lysis buffer was added and mixed for 2 min

Upstream tips
- 50,000 cells/well were seeded
Protocol tips
- Cells werre treated with α-TOS, NTα-TOS-NPs, or T-α-TOS-NPs for 12 h at 37 °C in 5% CO2. - RIPA cell lysis buffer was added and mixed for 2 min

Publication protocol

Intracellular ROS was assessed by the ROS assay Kit from Cell Biolabs. MCF-7 and H9C2 cells (50,000 cells/well) were plated in 96-well plates in 100 µL medium and grown for overnight. Then the medium was replenished and cells were treated with α-TOS, NTα-TOS-NPs, or T-α-TOS-NPs (40 µM for MCF-7, 60 µM for H9C2 with respect to α-TOS) for 12 h at 37 °C in 5% CO2 atmosphere. As a control, oligomycin (25 µM) treatment was performed. The media was removed and 100 µL of 1X dichloro-dihydro-fluorescein diacetate (DCFH-DA)/media solution was added to the cells and incubated for 45 min. The solution was removed and washed with PBS twice. Finally, RIPA cell lysis buffer was added and mixed for 2 min. The fluorescence was recorded at 485/530 nm.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - H9c2 rat cardiomyocytes using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - H9c2 rat cardiomyocytes using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.